Supplemental material

The tegument protein UL71 of human cytomegalovirus is involved in late envelopment and affects multivesicular bodies


Files in this Data Supplement:

Supplemental file 1 - Movie S1. Electron tomography of a section of the assembly compartment of a TB71stop virus infected cell. The video shows an animation through a z-series of digital slices, reconstructed from a tilt series taken from a 500 nm thick section of a TBstop71 infected HFF 5 days postinfection. Note that the majority of TBstop71 virus particles is in a process of budding. The usage of enlarged vesicles as a multiple budding site for numerous capsids is even more obvious in the reconstructed video than on ultrathin sections. Note areas with high mass density such as membranes occur bright in the dark field STEM tomograms.

Download avi file (29Mb)

J. Virol. Schauflinger et al.

Publikationsliste Institut für Virologie ab 2000

2016Two Cases of Vaccine-Derived Poliovirus Infection in an Oncology Ward. N Engl J Med. 2016;374(13): 1296-8 (Impact(2015)=59.558, Typ=Letter; Letter; Case Reports)
Schubert A, Böttcher S, Eis-Hübinger AM
2016Generation of a Gaussia luciferase-expressing endotheliotropic cytomegalovirus for screening approaches and mutant analyses. J Virol Methods. 2016;235: 182-9 (Impact(2015)=1.508, Typ=Article; Journal Article)
Falk JJ, Laib Sampaio K, Stegmann C, Lieber D, Kropff B, Mach M, Sinzger C
2016Tetraspanin CD151 Promotes Initial Events in Human Cytomegalovirus Infection. J Virol. 2016;90(14): 6430-42 (Impact(2015)=4.606, Typ=Article; Journal Article)
Hochdorfer D, Florin L, Sinzger C, Lieber D
2016Regulatory Interaction between the Cellular Restriction Factor IFI16 and Viral pp65 (pUL83) Modulates Viral Gene Expression and IFI16 Protein Stability. J Virol. 2016;90(18): 8238-50 (Impact(2015)=4.606, Typ=Article; Journal Article)
Biolatti M, Dell'Oste V, Pautasso S, von Einem J, Marschall M, Plachter B, Gariglio M, De Andrea M, Landolfo S
2016Characterization of Cytomegalovirus Breakthrough Events in a Phase 2 Prophylaxis Trial of Letermovir (AIC246, MK 8228). J Infect Dis. 2016;213(1): 23-30 (Impact(2015)=6.344, Typ=Article; Journal Article)
Lischka P, Michel D, Zimmermann H
2016Phenotypic characterization of human cytomegalovirus strains in cell cultures based on their transmission kinetics. J Gen Virol. 2016;97(9): 2376-86 (Impact(2015)=3.192, Typ=Article; Journal Article)
Subramanian N, Wu Z, Mertens TM
2016The contribution of pUL74 to growth of human cytomegalovirus is masked in the presence of RL13 and UL128 expression. J Gen Virol. 2016;97(8): 1917-27 (Impact(2015)=3.192, Typ=Journal Article)
Laib Sampaio K, Stegmann C, Brizic I, Adler B, Stanton RJ, Sinzger C
2016Megapinocytosis: a novel endocytic pathway. Histochem Cell Biol. 2016;145(6): 617-27 (Impact(2015)=2.78, Typ=Article; Journal Article)
Bauer A, Subramanian N, Villinger C, Frascaroli G, Mertens T, Walther P
2016Inactivation and Environmental Stability of Zika Virus. Emerg Infect Dis. 2016;22(9): 1685-7 (Impact(2015)=6.994, Typ=Letter; Letter)
Müller JA, Harms M, Schubert A, Jansen S, Michel D, Mertens T, Schmidt-Chanasit J, Münch J
2016Recombinant Enterovirus A71 Subgenogroup C1 Strains, Germany, 2015. Emerg Infect Dis. 2016;22(10): 1843-6 (Impact(2015)=6.994, Typ=Letter; Letter)
Böttcher S, Obermeier PE, Neubauer K, Diedrich S, Laboratory Network for Enterovirus Diagnostics
2016Identification of resistance-associated HCMV UL97- and UL54-mutations and a UL97-polymporphism with impact on phenotypic drug-resistance. Antiviral Res. 2016;131: 1-8 (Impact(2015)=4.909, Typ=Article; Journal Article)
Fischer L, Imrich E, Sampaio KL, Hofmann J, Jahn G, Hamprecht K, Göhring K
2015A molecular tweezer antagonizes seminal amyloids and HIV infection. eLife. 2015;4 (Impact(2015)=8.282, Typ=Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural)
Lump E, Castellano LM, Meier C, Seeliger J, Erwin N, Sperlich B, Stürzel CM, Usmani S, Hammond RM, von Einem J, Gerold G, Kreppel F, Bravo-Rodriguez K, Pietschmann T, Holmes VM, Palesch D, Zirafi O, Weissman D, Sowislok A, Wettig B, Heid C, Kirchhoff F, Weil T, Klärner FG, Schrader T, Bitan G, Sanchez-Garcia E, Winter R, Shorter J, Münch J
20153D Analysis of HCMV Induced-Nuclear Membrane Structures by FIB/SEM Tomography: Insight into an Unprecedented Membrane Morphology. Viruses. 2015;7(11): 5686-704 (Impact(2015)=3.042, Typ=Journal Article)
Villinger C, Neusser G, Kranz C, Walther P, Mertens T
2015The Cellular Proteins Grb2 and DDX3 Are Increased upon Human Cytomegalovirus Infection and Act in a Proviral Fashion. PLoS ONE. 2015;10(6): e0131614 (Impact(2015)=3.057, Typ=Journal Article; Research Support, Non-U.S. Gov't)
Cavignac Y, Lieber D, Laib Sampaio K, Madlung J, Lamkemeyer T, Jahn G, Nordheim A, Sinzger C
2015Natural killer cells can inhibit the transmission of human cytomegalovirus in cell culture by using mechanisms from innate and adaptive immune responses.J Virol. 2015;89(5): 2906-17 (Impact(2015)=4.606, Typ=Journal Article)
Wu Z, Sinzger C, Reichel JJ, Just M, Mertens T
2015Interleukin-2 from Adaptive T Cells Enhances Natural Killer Cell Activity against Human Cytomegalovirus-Infected Macrophages.J Virol. 2015;89(12): 6435-41 (Impact(2015)=4.606, Typ=Journal Article; Research Support, Non-U.S. Gov't)
Wu Z, Frascaroli G, Bayer C, Schmal T, Mertens T
2015Human Cytomegalovirus pUL47 Modulates Tegumentation and Capsid Accumulation at the Viral Assembly Complex.J Virol. 2015;89(14): 7314-28 (Impact(2015)=4.606, Typ=Journal Article; Research Support, Non-U.S. Gov't)
Cappadona I, Villinger C, Schutzius G, Mertens T, von Einem J
2015A permanently growing human endothelial cell line supports productive infection with human cytomegalovirus under conditional cell growth arrest.Biotechniques. 2015;59(3): 127-36 (Impact(2015)=2.298, Typ=Journal Article; Research Support, Non-U.S. Gov't)
Lieber D, Hochdorfer D, Stoehr D, Schubert A, Lotfi R, May T, Wirth D, Sinzger C
2014

Single nucleotide polymorphisms of thymidine kinase and DNA polymerase genes in clinical herpes simplex virus type 1 isolates associated with different resistance phenotypes.

Schubert A, Gentner E, Bohn K, Schwarz M, Mertens T, Sauerbrei A.

Antiviral Res. 2014 Jul;107:16-22. doi: 10.1016/j.antiviral.2014.03.015. Epub 2014 Apr 18.

2014

Human cytomegalovirus infection impairs endothelial cell chemotaxis by disturbing VEGF signalling and actin polymerization.

Reinhardt B, Godfrey R, Fellbrich G, Frank H, Lüske A, Olieslagers S, Mertens T, Waltenberger J.

Cardiovasc Res. 2014 Nov 1;104(2):315-25. doi: 10.1093/cvr/cvu204. Epub 2014 Sep 16.

2014

Widespread context dependency of microRNA-mediated regulation.

Erhard F, Haas J, Lieber D, Malterer G, Jaskiewicz L, Zavolan M, Dölken L, Zimmer R.

Genome Res. 2014 Jun;24(6):906-19. doi: 10.1101/gr.166702.113. Epub 2014 Mar 25.

2014

Associations of fatty liver disease and other factors affecting serum SHBG concentrations: a population based study on 1657 subjects.

Flechtner-Mors M, Schick A, Oeztuerk S, Haenle MM, Wilhelm M, Koenig W, Imhof A, Boehm BO, Graeter T, Mason RA, Kratzer W, Akinli AS; EMIL-Study Group.

Horm Metab Res. 2014 Apr;46(4):287-93. doi: 10.1055/s-0033-1354369. Epub 2013 Sep 2.

2014

Acute liver failure in an immunocompetent patient.

Paula VS, Silva AP, Michel D, Melgaço JG, Vieira YR, Santos DC, Araújo CC, Pacheco-Moreira LF, Pelajo-Machado M, Nascimento CM, Schmidt-Chanasit J, Pinto MA.

J Clin Virol. 2014 May;60(1):1-3. doi: 10.1016/j.jcv.2014.03.008. Epub 2014 Mar 22. No abstract available.

2014

Cytomegalovirus infection impairs immunosuppressive and antimicrobial effector functions of human multipotent mesenchymal stromal cells.

Meisel R, Heseler K, Nau J, Schmidt SK, Leineweber M, Pudelko S, Wenning J, Zimmermann A, Hengel H, Sinzger C, Degistirici Ö, Sorg RV, Däubener W.

Mediators Inflamm. 2014;2014:898630. doi: 10.1155/2014/898630. Epub 2014 Mar 23.

2014

Distinct properties of human cytomegalovirus strains and the appropriate choice of strains for particular studies.

Frascaroli G, Sinzger C.

Methods Mol Biol. 2014;1119:29-46. doi: 10.1007/978-1-62703-788-4_3.

2014

Polymerase chain reaction and Southern blot-based analysis of the C9orf72 hexanucleotide repeat in different motor neuron diseases.

Hübers A, Marroquin N, Schmoll B, Vielhaber S, Just M, Mayer B, Högel J, Dorst J, Mertens T, Just W, Aulitzky A, Wais V, Ludolph AC, Kubisch C, Weishaupt JH, Volk AE.

Neurobiol Aging. 2014 May;35(5):1214.e1-6. doi: 10.1016/j.neurobiolaging.2013.11.034. Epub 2013 Dec 4.

2014

Preemptive treatment of Cytomegalovirus infection in kidney transplant recipients with letermovir: results of a Phase 2a study.

Stoelben S, Arns W, Renders L, Hummel J, Mühlfeld A, Stangl M, Fischereder M, Gwinner W, Suwelack B, Witzke O, Dürr M, Beelen DW, Michel D, Lischka P, Zimmermann H, Rübsamen-Schaeff H, Budde K.

Transpl Int. 2014 Jan;27(1):77-86. doi: 10.1111/tri.12225. Erratum in: Transpl Int. 2014 Apr;27(4):416.

2013

Generation and characterization of a GCV resistant HCMV UL97-mutation and a drug sensitive UL54-mutation.

Fischer L, Laib Sampaio K, Jahn G, Hamprecht K, Göhring K.

Antiviral Res. 2013 Dec;100(3):575-7. doi: 10.1016/j.antiviral.2013.09.026. Epub 2013 Oct 10.

2013

Late HBsAg seroreversion of mutated hepatitis B virus after bone marrow transplantation.

Schubert A, Michel D, Mertens T.

BMC Infect Dis. 2013 May 16;13:223. doi: 10.1186/1471-2334-13-223.

2013

Fast selection of maribavir resistant cytomegalovirus in a bone marrow transplant recipient.

Schubert A, Ehlert K, Schuler-Luettmann S, Gentner E, Mertens T, Michel D.

BMC Infect Dis. 2013 Jul 19;13:330. doi: 10.1186/1471-2334-13-330.

2013

Background paper to the recommendation for the preferential use of live-attenuated influenza vaccine in children aged 2-6 years in Germany.

Falkenhorst G, Harder T, Remschmidt C, Terhardt M, Zepp F, Ledig T, Wicker S, Keller-Stanislawski B, Mertens T.

Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2013 Nov;56(11):1557-64. doi: 10.1007/s00103-013-1844-9. Erratum in: Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2014 Apr;57(4):473-4.

2013

[Concepts, effectiveness, and perspectives of pandemic and seasonal influenza vaccines].

Grund S, Wichmann O, Mertens T, Hengel H.

Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2013 Jan;56(1):76-86. doi: 10.1007/s00103-012-1590-4. German.

2013

Background paper to the recommendation for routine rotavirus vaccination of infants in Germany.

Koch J, Wiese-Posselt M, Remschmidt C, Wichmann O, Bertelsmann H, Garbe E, Hengel H, Meerpohl JJ, Mas Marques A, Oppermann H, Hummers-Pradier E, von Kries R, Mertens T.
2013

Analysis of human cytomegalovirus secondary envelopment by advanced electron microscopy.

Schauflinger M, Villinger C, Mertens T, Walther P, von Einem J.

Cell Microbiol. 2013 Feb;15(2):305-14. doi: 10.1111/cmi.12077. Epub 2012 Dec 20.

2013

Influenza vaccination and the end of simplicity.

Mertens T.

Dtsch Arztebl Int. 2013 Nov 22;110(47):791-2. doi: 10.3238/arztebl.2013.0791. No abstract available.

2013

Human cytomegalovirus infection of M1 and M2 macrophages triggers inflammation and autologous T-cell proliferation.

Bayer C, Varani S, Wang L, Walther P, Zhou S, Straschewski S, Bachem M, Söderberg-Naucler C, Mertens T, Frascaroli G.

J Virol. 2013 Jan;87(1):67-79. doi: 10.1128/JVI.01585-12. Epub 2012 Oct 10.

2013

Nuclear targeting of human cytomegalovirus large tegument protein pUL48 is essential for viral growth.

Brock I, Krüger M, Mertens T, von Einem J.

J Virol. 2013 May;87(10):6005-19. doi: 10.1128/JVI.03558-12. Epub 2013 Mar 20.

2013

The ULb' region of the human cytomegalovirus genome confers an increased requirement for the viral protein kinase UL97.

Wang D, Li G, Schauflinger M, Nguyen CC, Hall ED, Yurochko AD, von Einem J, Kamil JP.

J Virol. 2013 Jun;87(11):6359-76. doi: 10.1128/JVI.03477-12. Epub 2013 Mar 27.

2013

Human cytomegalovirus-induced NKG2C(hi) CD57(hi) natural killer cells are effectors dependent on humoral antiviral immunity.

Wu Z, Sinzger C, Frascaroli G, Reichel J, Bayer C, Wang L, Schirmbeck R, Mertens T.

J Virol. 2013 Jul;87(13):7717-25. doi: 10.1128/JVI.01096-13. Epub 2013 May 1.

2013

Detection of protein interactions during virus infection by bimolecular fluorescence complementation.

Becker S, von Einem J.

Methods Mol Biol. 2013;1064:29-41. doi: 10.1007/978-1-62703-601-6_3.

2013

Determination of HSV-1 infectivity by plaque assay and a luciferase reporter cell line.

Lieber D, Bailer SM.

Methods Mol Biol. 2013;1064:171-81. doi: 10.1007/978-1-62703-601-6_12.

2013

Generation of a stable cell line for constitutive miRNA expression.

Lieber D.

Methods Mol Biol. 2013;1064:183-200. doi: 10.1007/978-1-62703-601-6_13.

2013

Applications for a dual fluorescent human cytomegalovirus in the analysis of viral entry.

Sampaio KL, Jahn G, Sinzger C.

Methods Mol Biol. 2013;1064:201-9. doi: 10.1007/978-1-62703-601-6_14.

2013

Analysis of cell migration during human cytomegalovirus (HCMV) infection.

Varani S, Frascaroli G.

Methods Mol Biol. 2013;1064:299-313. doi: 10.1007/978-1-62703-601-6_22.

2013

Assessment of natural killer cell responses to human cytomegalovirus-infected macrophages.

Wu Z, Frascaroli G, Mertens T.

Methods Mol Biol. 2013;1064:289-98. doi: 10.1007/978-1-62703-601-6_21.

2013

Cytomegalovirus impairs the induction of indoleamine 2,3-dioxygenase mediated antimicrobial and immunoregulatory effects in human fibroblasts.

Heseler K, Schmidt SK, Spekker K, Sinzger C, Sorg RV, Quambusch M, Zimmermann A, Meisel R, Däubener W.

PLoS One. 2013 May 15;8(5):e64442. doi: 10.1371/journal.pone.0064442. Print 2013.

2013

CD14-dependent monocyte isolation enhances phagocytosis of listeria monocytogenes by proinflammatory, GM-CSF-derived macrophages.

Neu C, Sedlag A, Bayer C, Förster S, Crauwels P, Niess JH, van Zandbergen G, Frascaroli G, Riedel CU.

PLoS One. 2013 Jun 11;8(6):e66898. doi: 10.1371/journal.pone.0066898. Print 2013.

2013

Human cytomegalovirus tegument protein pp150 acts as a cyclin A2-CDK-dependent sensor of the host cell cycle and differentiation state.

Bogdanow B, Weisbach H, von Einem J, Straschewski S, Voigt S, Winkler M, Hagemeier C, Wiebusch L.

Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17510-5. doi: 10.1073/pnas.1312235110. Epub 2013 Oct 7.

2012

Report on the 1st international workshop on procedures for the development of evidence-based vaccination recommendations, Berlin, Germany, 22-23 November 2010.

Matysiak-Klose D, Ahmed F, Duclos P, Falck-Ytter Y, Forland F, Houweling H, Kramarz P, Langley JM, Mertens T, Schünemann H, Senouci K, Temte J, Wichmann O.

Vaccine. 2012 Mar 23;30(14):2399-404. doi: 10.1016/j.vaccine.2011.12.004. Epub 2011 Dec 13.

2012NEX-TRAP, a novel method for in vivo analysis of nuclear export of proteins.

Raschbichler V, Lieber D, Bailer SM.

Traffic. 2012 Oct;13(10):1326-34. doi: 10.1111/j.1600-0854.2012.01389.x. Epub 2012 Aug 1.

2012A beta-herpesvirus with fluorescent capsids to study transport in living cells.

Bosse JB, Bauerfeind R, Popilka L, Marcinowski L, Taeglich M, Jung C, Striebinger H, von Einem J, Gaul U, Walther P, Koszinowski UH, Ruzsics Z.

PLoS One. 2012;7(7):e40585. doi: 10.1371/journal.pone.0040585. Epub 2012 Jul 11.

2012Antiviral function and efficacy of polyvalent immunoglobulin products against CMV isolates in different human cell lines.

Frenzel K, Ganepola S, Michel D, Thiel E, Krüger DH, Uharek L, Hofmann J.

Med Microbiol Immunol. 2012 Aug;201(3):277-86. doi: 10.1007/s00430-012-0229-2.

2012Mutational mapping of pUL131A of human cytomegalovirus emphasizes its central role for endothelial cell tropism.

Schuessler A, Sampaio KL, Straschewski S, Sinzger C.

J Virol. 2012 Jan;86(1):504-12. doi: 10.1128/JVI.05354-11. Epub 2011 Oct 26.

2012A leucine zipper motif of a tegument protein triggers final envelopment of human cytomegalovirus.

Meissner CS, Suffner S, Schauflinger M, von Einem J, Bogner E.

J Virol. 2012 Mar;86(6):3370-82. doi: 10.1128/JVI.06556-11. Epub 2011 Dec 28.

2012Antiviral treatment of cytomegalovirus infection: an update.

Härter G, Michel D.

Expert Opin Pharmacother. 2012 Apr;13(5):623-7. doi: 10.1517/14656566.2012.658775. Epub 2012 Feb 2.

2012Influenza A(H1N1)pdm09 antibodies after pandemic and trivalent seasonal influenza vaccination as well as natural infection in November 2010 in Hamburg, Germany.

Cramer J, Mac T, Hogan B, Stauga S, Eberhardt S, Wichmann O, Mertens T, Burchard G.

Euro Surveill. 2012 Jan 12;17(2). pii: 20052.

2012Molecular basis of early epithelial response to streptococcal exotoxin: role of STIM1 and Orai1 proteins.

Usmani SM, von Einem J, Frick M, Miklavc P, Mayenburg M, Husmann M, Dietl P, Wittekindt OH.

Cell Microbiol. 2012 Mar;14(3):299-315. doi: 10.1111/j.1462-5822.2011.01724.x. Epub 2011 Dec 13.

2012[Prevention of nosocomial transmission of human immunodeficiency virus (HIV) from HIV-positive healthcare workers. Recommendations of the German Association for the Control of Viral Diseases (DVV) e.V. and the Society for Virology (GfV) e.V].

Rabenau HF, Gottschalk R, Gürtler L, Haberl AE, Hamouda O, Himmelreich H, Korn K, Mertens T, Schmidt KW, Schmiedel S, Spickhoff A, Wirz G, Wutzler P, Wicker S.

Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2012 Aug;55(8):937-43. doi: 10.1007/s00103-012-1546-8. German.

2012The 7-transmembrane protein homologue UL78 of the human cytomegalovirus forms oligomers and traffics between the plasma membrane and different intracellular compartments.

Wagner S, Arnold F, Wu Z, Schubert A, Walliser C, Tadagaki K, Jockers R, Mertens T, Michel D.

Arch Virol. 2012 May;157(5):935-49. doi: 10.1007/s00705-012-1246-6. Epub 2012 Feb 12.

2011

The tegument protein UL71 of human cytomegalovirus is involved in late envelopment and affects multivesicular bodies.

Schauflinger M, Fischer D, Schreiber A, Chevillotte M, Walther P, Mertens T, von Einem J.

J Virol. 2011 Apr;85(8):3821-32. Epub 2011 Feb 2.

2011

M94 is essential for the secondary envelopment of murine cytomegalovirus.

Maninger S, Bosse JB, Lemnitzer F, Pogoda M, Mohr CA, von Einem J, Walther P, Koszinowski UH, Ruzsics Z.

J Virol. 2011 Sep;85(18):9254-67. Epub 2011 Jun 29.

2011

UL74 of human cytomegalovirus reduces the inhibitory effect of gH-specific and gB-specific antibodies.

Jiang XJ, Sampaio KL, Ettischer N, Stierhof YD, Jahn G, Kropff B, Mach M, Sinzger C.

Arch Virol. 2011 Dec;156(12):2145-55. Epub 2011 Sep 22.

2011

Adoptive transfer and selective reconstitution of streptamer-selected cytomegalovirus-specific CD8+ T cells leads to virus clearance in patients after allogeneic peripheral blood stem cell transplantation.

Schmitt A, Tonn T, Busch DH, Grigoleit GU, Einsele H, Odendahl M, Germeroth L, Ringhoffer M, Ringhoffer S, Wiesneth M, Greiner J, Michel D, Mertens T, Rojewski M, Marx M, von Harsdorf S, Döhner H, Seifried E, Bunjes D, Schmitt M.

Transfusion. 2011 Mar;51(3):591-9. doi: 10.1111/j.1537-2995.2010.02940.x. Epub 2010 Dec 6.

2011

Post-pandemic seroprevalence of pandemic influenza A (H1N1) 2009 infection (swine flu) among children <18 years in Germany.

von Kries R, Weiss S, Falkenhorst G, Wirth S, Kaiser P, Huppertz HI, Tenenbaum T, Schroten H, Streng A, Liese J, Shai S, Niehues T, Girschick H, Kuscher E, Sauerbrey A, Peters J, Wirsing von König CH, Rückinger S, Hampl W, Michel D, Mertens T.

PLoS One. 2011;6(9):e23955. Epub 2011 Sep 7.

2011

Protein pUL128 of human cytomegalovirus is necessary for monocyte infection and blocking of migration.

Straschewski S, Patrone M, Walther P, Gallina A, Mertens T, Frascaroli G.

J Virol. 2011 May;85(10):5150-8. Epub 2011 Mar 2.

2011

Plasmodium falciparum-infected erythrocytes and beta-hematin induce partial maturation of human dendritic cells and increase their migratory ability in response to lymphoid chemokines

Giusti P, Urban BC, Frascaroli G, Albrecht L, Tinti A, Troye-Blomberg M, Varani S.

Infect Immun. 2011 Jul;79(7):2727-36. Epub 2011 Apr 4.

2011

The novel anti-rheumatic compound Rabeximod impairs differentiation and function of human pro-inflammatory dendritic cells and macrophages

Giusti P, Frascaroli G, Tammik C, Gredmark-Russ S, Söderberg-Nauclér C, Varani S.

Immunobiology. 2011 Jan-Feb;216(1-2):243-50. Epub 2010 Apr 28.

2011

The immunodominant CD8 T cell response to the human cytomegalovirus tegument phosphoprotein pp65(495-503) epitope critically depends on CD4 T cell help in vaccinated HLA-A*0201 transgenic mice.

Reiser M, Wieland A, Plachter B, Mertens T, Greiner J, Schirmbeck R.

J Immunol. 2011 Sep 1;187(5):2172-80. Epub 2011 Aug 1.

2011

Telomerase gene mutations are associated with cirrhosis formation.

Hartmann D, Srivastava U, Thaler M, Kleinhans KN, N'kontchou G, Scheffold A, Bauer K, Kratzer RF, Kloos N, Katz SF, Song Z, Begus-Nahrmann Y, Kleger A, von Figura G, Strnad P, Lechel A, Günes C, Potthoff A, Deterding K, Wedemeyer H, Ju Z, Song G, Xiao F, Gillen S, Schrezenmeier H, Mertens T, Ziol M, Friess H, Jarek M, Manns MP, Beaugrand M, Rudolph KL.

Hepatology. 2011 May;53(5):1608-17. doi: 10.1002/hep.24217.

2010

Impact of ETIF deletion on safety and immunogenicity of equine herpesvirus type 1-vectored vaccines.

Hofmann-Sieber H, Wild J, Fiedler N, Tischer K, von Einem J, Osterrieder N, Hofmann H, Köstler J, Wagner R.

J Virol. 2010 Nov;84(22):11602-13. Epub 2010 Sep 8.

2010

Equine herpesvirus type 1 (EHV-1) utilizes microtubules, dynein, and ROCK1 to productively infect cells.

Frampton AR Jr, Uchida H, von Einem J, Goins WF, Grandi P, Cohen JB, Osterrieder N, Glorioso JC.

Vet Microbiol. 2010 Feb 24;141(1-2):12-21. Epub 2009 Aug 8.

2010

A new tool linking human cytomegalovirus drug resistance mutations to resistance phenotypes.

Chevillotte M, von Einem J, Meier BM, Lin FM, Kestler HA, Mertens T.

Antiviral Res. 2010 Feb;85(2):318-27. Epub 2009 Oct 22. Review.

2010

Human cytomegaloviruses expressing yellow fluorescent fusion proteins--characterization and use in antiviral screening.

Straschewski S, Warmer M, Frascaroli G, Hohenberg H, Mertens T, Winkler M.

PLoS One. 2010 Feb 11;5(2):e9174.

2010

Intravenous zanamivir for patients with pneumonitis due to pandemic (H1N1) 2009 influenza virus.

Härter G, Zimmermann O, Maier L, Schubert A, Mertens T, Kern P, Wöhrle J.

Clin Infect Dis. 2010 May 1;50(9):1249-51.

2010

Polyomavirus BK-specific CD8+ T cell responses in patients after allogeneic stem cell transplant.

Schneidawind D, Schmitt A, Wiesneth M, Mertens T, Bunjes D, Freund M, Schmitt M.

Leuk Lymphoma. 2010 Jun;51(6):1055-62.

2010

Differentiation between polymorphisms and resistance-associated mutations in human cytomegalovirus DNA polymerase.

Chevillotte M, Ersing I, Mertens T, von Einem J.

Antimicrob Agents Chemother. 2010 Dec;54(12):5004-11. Epub 2010 Sep 27.

2010

Improved detection of mutated human cytomegalovirus UL97 by pyrosequencing.

Schindele B, Apelt L, Hofmann J, Nitsche A, Michel D, Voigt S, Mertens T, Ehlers B.

Antimicrob Agents Chemother. 2010 Dec;54(12):5234-41. Epub 2010 Sep 27.

2009

Genome-wide innate immune responses in HIV-1-infected macrophages are preserved despite attenuation of the NF-kappa B activation pathway.

Noursadeghi M, Tsang J, Miller RF, Straschewski S, Kellam P, Chain BM, Katz DR.

J Immunol. 2009 Jan 1;182(1):319-28.

2009

Toward an AIDS vaccine: lessons from natural simian immunodeficiency virus infections of African nonhuman primate hosts.

Sodora DL, Allan JS, Apetrei C, Brenchley JM, Douek DC, Else JG, Estes JD, Hahn BH, Hirsch VM, Kaur A, Kirchhoff F, Muller-Trutwin M, Pandrea I, Schmitz JE, Silvestri G.

Nat Med. 2009 Aug;15(8):861-5. Review.

2009

Is the high virulence of HIV-1 an unfortunate coincidence of primate lentiviral evolution?

Kirchhoff F.

Nat Rev Microbiol. 2009 Jun;7(6):467-76. Epub 2009 Mar 23.

2009

Tetherin-driven adaptation of Vpu and Nef function and the evolution of pandemic and nonpandemic HIV-1 strains.

Sauter D, Schindler M, Specht A, Landford WN, Münch J, Kim KA, Votteler J, Schubert U, Bibollet-Ruche F, Keele BF, Takehisa J, Ogando Y, Ochsenbauer C, Kappes JC, Ayouba A, Peeters M, Learn GH, Shaw G, Sharp PM, Bieniasz P, Hahn BH, Hatziioannou T, Kirchhoff F.

Cell Host Microbe. 2009 Nov 19;6(5):409-21.

2009

The inability to disrupt the immunological synapse between infected human T cells and APCs distinguishes HIV-1 from most other primate lentiviruses.

Arhel N, Lehmann M, Clauss K, Nienhaus GU, Piguet V, Kirchhoff F.

J Clin Invest. 2009 Oct;119(10):2965-75. doi: 10.1172/JCI38994. Epub 2009 Sep 14.

2009

Nef proteins from simian immunodeficiency viruses are tetherin antagonists.

Zhang F, Wilson SJ, Landford WC, Virgen B, Gregory D, Johnson MC, Munch J, Kirchhoff F, Bieniasz PD, Hatziioannou T.

Cell Host Microbe. 2009 Jul 23;6(1):54-67. Epub 2009 Jun 4.

2009

Fibrils of prostatic acid phosphatase fragments boost infections with XMRV (xenotropic murine leukemia virus-related virus), a human retrovirus associated with prostate cancer.

Hong S, Klein EA, Das Gupta J, Hanke K, Weight CJ, Nguyen C, Gaughan C, Kim KA, Bannert N, Kirchhoff F, Munch J, Silverman RH.

J Virol. 2009 Jul;83(14):6995-7003. Epub 2009 Apr 29.

2009

Conservation of Nef function across highly diverse lineages of SIVsmm.

Schmökel J, Li H, Bailes E, Schindler M, Silvestri G, Hahn BH, Apetrei C, Kirchhoff F.

Retrovirology. 2009 Apr 9;6:36.

2009

CD95 co-stimulation blocks activation of naive T cells by inhibiting T cell receptor signaling.

Strauss G, Lindquist JA, Arhel N, Felder E, Karl S, Haas TL, Fulda S, Walczak H, Kirchhoff F, Debatin KM.

J Exp Med. 2009 Jun 8;206(6):1379-93. Epub 2009 Jun 1.

2009

The cationic properties of SEVI underlie its ability to enhance human immunodeficiency virus infection.

Roan NR, Münch J, Arhel N, Mothes W, Neidleman J, Kobayashi A, Smith-McCune K, Kirchhoff F, Greene WC.

J Virol. 2009 Jan;83(1):73-80. Epub 2008 Oct 22.

2009

Antiviral treatment of cytomegalovirus infection and resistant strains.

Schreiber A, Härter G, Schubert A, Bunjes D, Mertens T, Michel D.

Expert Opin Pharmacother. 2009 Feb;10(2):191-209. Review.

2009

The human cytomegalovirus-encoded chemokine receptor US28 promotes angiogenesis and tumor formation via cyclooxygenase-2.

Maussang D, Langemeijer E, Fitzsimons CP, Stigter-van Walsum M, Dijkman R, Borg MK, Slinger E, Schreiber A, Michel D, Tensen CP, van Dongen GA, Leurs R, Smit MJ.

Cancer Res. 2009 Apr 1;69(7):2861-9. Epub 2009 Mar 24.

2009

Pharmacological and biochemical characterization of human cytomegalovirus-encoded G protein-coupled receptors.

Maussang D, Vischer HF, Schreiber A, Michel D, Smit MJ.

Methods Enzymol. 2009;460:151-71.

2009

Fluorescence-based assay for phenotypic characterization of human cytomegalovirus polymerase mutations regarding drug susceptibility and viral replicative fitness.

Chevillotte M, Schubert A, Mertens T, von Einem J.

Antimicrob Agents Chemother. 2009 Sep;53(9):3752-61. Epub 2009 Jun 22.

2009

Human cytomegalovirus paralyzes macrophage motility through down-regulation of chemokine receptors, reorganization of the cytoskeleton, and release of macrophage migration inhibitory factor.

Frascaroli G, Varani S, Blankenhorn N, Pretsch R, Bacher M, Leng L, Bucala R, Landini MP, Mertens T.

J Immunol. 2009 Jan 1;182(1):477-88.

2009

Major tegument protein pp65 of human cytomegalovirus is required for the incorporation of pUL69 and pUL97 into the virus particle and for viral growth in macrophages.

Chevillotte M, Landwehr S, Linta L, Frascaroli G, Lüske A, Buser C, Mertens T, von Einem J.

J Virol. 2009 Mar;83(6):2480-90. Epub 2008 Dec 30.

2009

Human cytomegalovirus targets different subsets of antigen-presenting cells with pathological consequences for host immunity: implications for immunosuppression, chronic inflammation and autoimmunity.

Varani S, Frascaroli G, Landini MP, Söderberg-Nauclér C.

Rev Med Virol. 2009 May;19(3):131-45. Review.

2009

Generalized Wegener's granulomatosis in an immunocompetent adult after cytomegalovirus mononucleosis and bacterial urinary tract infection.

Varani S, Mastroianni A, Frascaroli G, Tammik C, Rahbar A, Christensson M, Rossini G, Landini MP, Söderberg-Nauclér C.

Arthritis Rheum. 2009 May;60(5):1558-62.

2008

Hantavirus outbreak, Germany, 2007.

Hofmann J, Meisel H, Klempa B, Vesenbeckh SM, Beck R, Michel D, Schmidt-Chanasit J, Ulrich RG, Grund S, Enders G, Kruger DH.

Emerg Infect Dis. 2008 May;14(5):850-2.

2008

Protein kinase inhibitors of the quinazoline class exert anti-cytomegaloviral activity in vitro and in vivo.

Schleiss M, Eickhoff J, Auerochs S, Leis M, Abele S, Rechter S, Choi Y, Anderson J, Scott G, Rawlinson W, Michel D, Ensminger S, Klebl B, Stamminger T, Marschall M.

Antiviral Res. 2008 Jul;79(1):49-61. Epub 2008 Feb 22.

2008

Ganciclovir/valganciclovir prophylaxis decreases cytomegalovirus-related events and bronchiolitis obliterans syndrome after lung transplantation

Chmiel C, Speich R, Hofer M, Michel D, Mertens T, Weder W, Boehler A.

Clin Infect Dis. 2008 Mar 15;46(6):831-9.

2008

Human cytomegalovirus infection and antiviral immunity in septic patients without canonical immunosuppression

von Müller L, Mertens T.

Med Microbiol Immunol. 2008 Jun;197(2):75-82. Epub 2008 Mar 8.

2008

Multimer staining of cytomegalovirus phosphoprotein 65-specific T cells for diagnosis and therapeutic purposes: a comparative study

Yao J, Bechter C, Wiesneth M, Härter G, Götz M, Germeroth L, Guillaume P, Hasan F, von Harsdorf S, Mertens T, Michel D, Döhner H, Bunjes D, Schmitt M, Schmitt A.

Clin Infect Dis. 2008 May 15;46(10):e96-105.

2008

Silencing HIV-1 In Vivo

Kirchhoff F.

Cell. 2008 Aug 22;134(4):566-8.

2008

Inefficient Nef-mediated downmodulation of CD3 and MHC-I correlates with loss of CD4+T cells in natural SIV infection

Schindler M, Schmökel J, Specht A, Li H, Münch J, Khalid M, Sodora DL, Hahn BH, Silvestri G, Kirchhoff F.

PLoS Pathog. 2008 Jul 18;4(7):e1000107.

2008

Human immunodeficiency virus type 1 nef expression prevents AP-2-mediated internalization of the major histocompatibility complex class II-associated invariant chain

Toussaint H, Gobert FX, Schindler M, Banning C, Kozik P, Jouve M, Kirchhoff F, Benaroch P.

J Virol. 2008 Sep;82(17):8373-82. Epub 2008 Jul 2.

2008

CD4 binding affinity determines human immunodeficiency virus type 1-induced alpha interferon production in plasmacytoid dendritic cells

Haupt S, Donhauser N, Chaipan C, Schuster P, Puffer B, Daniels RS, Greenough TC, Kirchhoff F, Schmidt B.

J Virol. 2008 Sep;82(17):8900-5. Epub 2008 Jun 25.

2008

Is Nef the elusive cause of HIV-associated hematopoietic dysfunction?

Kirchhoff F, Silvestri G.

J Clin Invest. 2008 May;118(5):1622-5.

2008

Selective downregulation of rhesus macaque and sooty mangabey major histocompatibility complex class I molecules by Nef alleles of simian immunodeficiency virus and human immunodeficiency virus type 2

DeGottardi MQ, Specht A, Metcalf B, Kaur A, Kirchhoff F, Evans DT.

J Virol. 2008 Mar;82(6):3139-46. Epub 2008 Jan 16.

2008

Selective downmodulation of HLA-A and -B by Nef alleles from different groups of primate lentiviruses

Specht A, DeGottardi MQ, Schindler M, Hahn B, Evans DT, Kirchhoff F.

Virology. 2008 Mar 30;373(1):229-37. Epub 2007 Dec 21.

2008

Role of Nef in primate lentiviral immunopathogenesis

Kirchhoff F, Schindler M, Specht A, Arhel N, Münch J.

Cell Mol Life Sci. 2008 Sep;65(17):2621-36.

2007

Pharmacokinetics and pharmacodynamics of mycophenolic acid after enteric-coated
mycophenolate versus mycophenolate mofetil in patients with progressive IgA
nephritis
.

Czock D, Rasche FM, Carius A, Glander P, Budde K, Bauer S, Keller F, von Müller L.

J Clin Pharmacol. 2007 Jul;47(7):850-9. Epub 2007 May 25.

2007

Human cytomegalovirus differentially controls B cell and T cell responses through
effects on plasmacytoid dendritic cells.

Varani S, Cederarv M, Feld S, Tammik C, Frascaroli G, Landini MP, Söderberg-Nauclér C.

J Immunol. 2007 Dec 1;179(11):7767-76.

2007

Sequential immunosuppressive therapy in progressive IgA nephropathy.

Rasche FM, Keller F, von Müller L, Czock D, Lepper PM.

Contrib Nephrol. 2007;157:109-13.

2007

Cytomegalovirus primary envelopment occurs at large infoldings of the inner
nuclear membrane.


Buser C, Walther P, Mertens T, Michel D.

J Virol. 2007 Mar;81(6):3042-8. Epub 2006 Dec 27.

2007

CMV monitoring using blood cells and plasma: a comparison of apples with oranges?

von Müller L, Hinz J, Bommer M, Hampl W, Kluwick S, Wiedmann M, Bunjes D, Mertens T.

Bone Marrow Transplant. 2007 Mar;39(6):353-7. Epub 2007 Feb 5.

2007

HCMV-infection in a human arterial organ culture model: effects on cell
proliferation and neointimal hyperplasia.


Voisard R, Krügers T, Reinhardt B, Vaida B, Baur R, Herter T, Lüske A, Weckermann D, Weingärtner K, Rössler W, Hombach V, Mertens T.

BMC Microbiol. 2007 Jul 20;7:68.

2007

Targeted release of oncolytic measles virus by blood outgrowth endothelial cells
in situ inhibits orthotopic gliomas.


Wei J, Wahl J, Nakamura T, Stiller D, Mertens T, Debatin KM, Beltinger C.

Gene Ther. 2007 Nov;14(22):1573-86. Epub 2007 Sep 27.

2007

Quantitative investigation of murine cytomegalovirus nucleocapsid interaction.

Buser C, Fleischer F, Mertens T, Michel D, Schmidt V, Walther P.

J Microsc. 2007 Oct;228(Pt 1):78-87.

2007

Cellular immunity and active human cytomegalovirus infection in patients with
septic shock.


von Muller L, Klemm A, Durmus N, Weiss M, Suger-Wiedeck H, Schneider M, Hampl W, Mertens T.

J Infect Dis. 2007 Nov 1;196(9):1288-95. Epub 2007 Oct 1.

2007

Primary human immunodeficiency virus type 1 nef alleles show major differences in
pathogenicity in transgenic mice.


Priceputu E, Hanna Z, Hu C, Simard MC, Vincent P, Wildum S, Schindler M, Kirchhoff F, Jolicoeur P.

J Virol. 2007 May;81(9):4677-93. Epub 2007 Feb 21.

2007

Human immunodeficiency virus type 1 variants resistant to first- and
second-version fusion inhibitors and cytopathic in ex vivo human lymphoid tissue.


Chinnadurai R, Rajan D, Münch J, Kirchhoff F.

J Virol. 2007 Jun;81(12):6563-72. Epub 2007 Apr 11.

2007

Discovery and optimization of a natural HIV-1 entry inhibitor targeting the gp41
fusion peptide.


Münch J, Ständker L, Adermann K, Schulz A, Schindler M, Chinnadurai R, Pöhlmann S, Chaipan C, Biet T, Peters T, Meyer B, Wilhelm D, Lu H, Jing W, Jiang S, Forssmann WG, Kirchhoff F.

Cell. 2007 Apr 20;129(2):263-75.

2007

Nef alleles from children with non-progressive HIV-1 infection modulate MHC-II
expression more efficiently than those from rapid progressors.


Schindler M, Wildum S, Casartelli N, Doria M, Kirchhoff F.

AIDS. 2007 May 31;21(9):1103-7.

2007

Proline 35 of human immunodeficiency virus type 1 (HIV-1) Vpr regulates the
integrity of the N-terminal helix and the incorporation of Vpr into virus
particles and supports the replication of R5-tropic HIV-1 in human lymphoid
tissue ex vivo.


Votteler J, Studtrucker N, Sörgel S, Münch J, Rücker E, Kirchhoff F, Schick B, Henklein P, Fossen T, Bruns K, Sharma A, Wray V, Schubert U.

J Virol. 2007 Sep;81(17):9572-6. Epub 2007 Jun 6.

2007

Conserved residue Lys574 in the cavity of HIV-1 Gp41 coiled-coil domain is
critical for six-helix bundle stability and virus entry.


He Y, Liu S, Jing W, Lu H, Cai D, Chin DJ, Debnath AK, Kirchhoff F, Jiang S.

J Biol Chem. 2007 Aug 31;282(35):25631-9. Epub 2007 Jul 6.

2007

Association of Nef with p21-activated kinase 2 is dispensable for efficient human
immunodeficiency virus type 1 replication and cytopathicity in ex vivo-infected
human lymphoid tissue.

Schindler M, Rajan D, Specht A, Ritter C, Pulkkinen K, Saksela K, Kirchhoff F.

J Virol. 2007 Dec;81(23):13005-14. Epub 2007 Sep 19.

2007

HIV infection in a patient with alpha-1 antitrypsin deficiency: a detrimental
combination?

Potthoff AV, Münch J, Kirchhoff F, Brockmeyer NH.

AIDS. 2007 Oct 1;21(15):2115-6

2007

Nef-mediated enhancement of virion infectivity and stimulation of viral
replication are fundamental properties of primate lentiviruses.


Münch J, Rajan D, Schindler M, Specht A, Rücker E, Novembre FJ, Nerrienet E, Müller-Trutwin MC, Peeters M, Hahn BH, Kirchhoff F.

J Virol. 2007 Dec;81(24):13852-64. Epub 2007 Oct 10.

2007

Semen-Derived Amyloid Fibrils Drastically Enhance HIV Infection.

Münch J, Rücker E, Ständker L, Adermann K, Goffinet C, Schindler M, Wildum S, Chinnadurai R, Rajan D, Specht A, Giménez-Gallego G, Sánchez PC, Fowler DM, Koulov A, Kelly JW, Mothes W, Grivel JC, Margolis L, Keppler OT, Forssmann WG, Kirchhoff F.

Cell. 2007 Dec 14;131(6):1059-71.

2007

A simian immunodeficiency virus V3 loop mutant that does not efficiently use CCR5
or common alternative coreceptors is moderately attenuated in vivo.


Pöhlmann S, Münch J, Aziz S, Reeves JD, Otto C, Leslie GJ, Hofmann H, Puffer BA, Baribaud F, Marzi A, Gramberg T, Chen Z, Stolte N, Haaft PT, Heeney JL, Stahl-Hennig C, Mätz-Rensing K, Schneider T, Doms RW, Kirchhoff F.

Virology. 2007 Apr 10;360(2):275-85. Epub 2006 Nov 28.

2006

Contribution of Vpu, Env, and Nef to CD4 down-modulation and resistance of human immunodeficiency virus type 1-infected T cells to superinfection.

Wildum S, Schindler M, Munch J, Kirchhoff F.

J Virol. 2006 Aug;80(16):8047-59.

2006

Nef-mediated suppression of T cell activation was lost in a lentiviral lineage that gave rise to HIV-1.

Schindler M, Munch J, Kutsch O, Li H, Santiago ML, Bibollet-Ruche F, Muller-Trutwin MC, Novembre FJ, Peeters M, Courgnaud V, Bailes E, Roques P, Sodora DL, Silvestri G, Sharp PM, Hahn BH, Kirchhoff F.

Cell. 2006 Jun 16;125(6):1055-67.

2006

Importance of the N-distal AP-2 binding element in Nef for simian immunodeficiency virus replication and pathogenicity in rhesus macaques.

Brenner M, Munch J, Schindler M, Wildum S, Stolte N, Stahl-Hennig C, Fuchs D, Matz-Rensing K, Franz M, Heeney J, Ten Haaft P, Swigut T, Hrecka K, Skowronski J, Kirchhoff F.

J Virol. 2006 May;80(9):4469-81.

2006

Effect of R77Q, R77A and R80A changes in Vpr on HIV-1 replication and CD4 T cell depletion in human lymphoid tissue ex vivo.

Rajan D, Wildum S, Rucker E, Schindler M, Kirchhoff F.

AIDS. 2006 Apr 4;20(6):831-6.

2006

Active cytomegalovirus infection in patients with septic shock.

von Muller L, Klemm A, Weiss M, Schneider M, Suger-Wiedeck H, Durmus N, Hampl W, Mertens T.

Emerg Infect Dis. 2006 Oct;12(10):1517-22.

2006

Human cytomegalovirus-induced reduction of extracellular matrix proteins in vascular smooth muscle cell cultures: a pathomechanism in vasculopathies?

Reinhardt B, Winkler M, Schaarschmidt P, Pretsch R, Zhou S, Vaida B, Schmid-Kotsas A, Michel D, Walther P, Bachem M, Mertens T.

J Gen Virol. 2006 Oct;87(Pt 10):2849-58.

2006

Human cytomegalovirus subverts the functions of monocytes, impairing chemokine-mediated migration and leukocyte recruitment.

Frascaroli G, Varani S, Moepps B, Sinzger C, Landini MP, Mertens T.

J Virol. 2006 Aug;80(15):7578-89.

2006

Severe graft rejection, increased immunosuppression, and active CMV infection in renal transplantation.

von Muller L, Schliep C, Storck M, Hampl W, Schmid T, Abendroth D, Mertens T.

J Med Virol. 2006 Mar;78(3):394-9.

2006

Mycophenolic acid therapy after cyclophosphamide pulses in progressive IgA nephropathy.

Rasche FM, Keller F, von Muller L, Sailer LK, Karges W, Czock D.

J Nephrol. 2006 Jul-Aug;19(4):465-72.

2006

Human cytomegalovirus-encoded chemokine receptor US28 promotes tumorigenesis.

Maussang D, Verzijl D, van Walsum M, Leurs R, Holl J, Pleskoff O, Michel D, van Dongen GA, Smit MJ.

Proc Natl Acad Sci U S A. 2006 Aug 29;103(35):13068-73. Epub 2006 Aug 21.

2006

Dendritic cell function in cytomegalovirus-infected patients with mononucleosis.

Frascaroli G, Varani S, Mastroianni A, Britton S, Gibellini D, Rossini G, Landini MP, Soderberg-Naucler C.

J Leukoc Biol. 2006 May;79(5):932-40. Epub 2006 Feb 24.

2006

Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli.

Tischer BK, von Einem J, Kaufer B, Osterrieder N.

Biotechniques. 2006 Feb;40(2):191-7.

2006

The alpha-TIF (VP16) homologue (ETIF) of equine herpesvirus 1 is essential for secondary envelopment and virus egress.

von Einem J, Schumacher D, O'Callaghan DJ, Osterrieder N.

J Virol. 2006 Mar;80(6):2609-20.

2005

RBP-Jkappa/SHARP recruits CtIP/CtBP corepressors to silence Notch target genes.

Oswald F, Winkler M, Cao Y, Astrahantseff K, Bourteele S, Knochel W, Borggrefe T

Mol Cell Biol. 2005 Dec;25(23):10379-90.

2005

Inhibition of HIV-1 group M and O isolates by fusion inhibitors.

Chinnadurai R, Munch J,
Dittmar MT, Kirchhoff F

AIDS. 2005 Nov 4;19(16):1919-22.

2005

The role of upstream U3 sequences in HIV-1 replication and CD4+ T cell depletion in human lymphoid tissue ex vivo.

Munch J, Rajan D, Rucker E, Wildum S, Adam N, Kirchhoff F

Virology. 2005 Oct 25;341(2):313-20. Epub 2005 Aug 15.

2005

Effect of naturally-occurring gp41 HR1 variations on susceptibility of HIV-1 to fusion inhibitors.

Chinnadurai R, Munch J, Kirchhoff F

AIDS. 2005 Sep 2;19(13):1401-5.

2005

LSECtin interacts with filovirus glycoproteins and the spike protein of SARS coronavirus.

Gramberg T, Hofmann H, Moller P, Lalor PF, Marzi A,
Geier M, Krumbiegel M, Winkler T, Kirchhoff F, Adams DH, Becker S, Munch J, Pohlmann S

Virology. 2005 Sep 30;340(2):224-36.

2005

Nef induces multiple genes involved in cholesterol synthesis and uptake in human immunodeficiency virus type 1-infected T cells.

van 't Wout AB, Swain JV, Schindler M, Rao U, Pathmajeyan MS, Mullins JI, Kirchhoff F

J Virol. 2005 Aug;79(15):10053-8.

2005

Primary sooty mangabey simian immunodeficiency virus and human immunodeficiency virus type 2 nef alleles modulate cell surface expression of various human receptors and enhance viral infectivity and replication.

Munch J, Schindler M, Wildum S, Rucker E, Bailer N, Knoop V, Novembre FJ,
Kirchhoff F

J Virol. 2005 Aug;79(16):10547-60.

2005

Nef proteins from diverse groups of primate lentiviruses downmodulate CXCR4 to inhibit migration to the chemokine stromal derived factor 1.

Hrecka K, Swigut T, Schindler M, Kirchhoff F, Skowronski J

J Virol. 2005 Aug;79(16):10650-9.

2005

HCMV infection of human vascular smooth muscle cells leads to enhanced expression of functionally intact PDGF beta-receptor.

Reinhardt B, Mertens T, Mayr-Beyrle U, Frank H, Luske A, Schierling K, Waltenberger J

Cardiovasc Res. 2005 Jul 1;67(1):151-60. Epub 2005 Apr 12.

2005

Fatal reactivation of postnatal cytomegalovirus infection with rapid emergence of ganciclovir resistance in an infant after allogeneic stem cell transplantation.

Michel D, Voigt S, Kershaw O, Kuhl JS, Mertens T, Ebell W, Meisel H

J Clin Microbiol. 2005 Jul;43(7):3551-4.

2005

Human immunodeficiency virus type 1 inhibits DNA damage-triggered apoptosis by a Nef-independent

Schindler M, Münch J, Kirchhoff F

J Virol. 2005 May;79(9):5489-98.

2005

Early selection of a new UL97 mutant with a severe defect of ganciclovir phosphorylation after valaciclovir prophylaxis and short-term ganciclovir therapy in a renal transplant recipient.

Hantz S, Michel D, Fillet AM,
Guigonis V, Champier G, Mazeron MC, Bensman A, Denis F, Mertens T, Dehee A, Alain S

Antimicrob Agents Chemother. 2005 Apr;49(4):1580-3.

2005

Human cytomegalovirus tegument protein ppUL35 is important for viral replication and particle formation.

Schierling K, Buser C, Mertens T, Winkler M

J Virol. 2005 Mar;79(5):3084-96.

2005

Human cytomegalovirus inhibits the migration of immature dendritic cells by down-regulating cell-surface CCR1 and CCR5.

Varani S, Frascaroli G, Homman-Loudiyi M, Feld S, Landini MP, Soderberg-Naucler C

J Leukoc Biol. 2005 Feb;77(2):219-28. Epub 2004 Nov 2.

2005

The human cytomegalovirus UL78 gene is highly conserved among clinical isolates, but is dispensable for replication in fibroblasts and a renal artery organ-culture system.

Michel D, Milotic I, Wagner M, Vaida B, Holl J, Ansorge R, Mertens T

J Gen Virol. 2005 Feb;86(Pt 2):297-306.

2005

Upregulation of functionally active vascular endothelial growth factor by human cytomegalovirus.

Reinhardt B, Schaarschmidt P, Bossert A, Luske A, Finkenzeller G, Michel D, Mertens T

J Gen Virol. 2005 Jan;86(Pt 1):23-30.

2005

Impaired dendritic cell immunophenotype and function in heart transplant patients undergoing active cytomegalovirus infection.

Frascaroli G, Varani S,
Gibellini D, Potena L, Lazzarotto T, Lemoli RM, Magelli C, Soderberg-Naucler C, Landini MP

Transplantation. 2005 Jan 27;79(2):219-27.

2004

Nef associates with p21-activated kinase 2 in a p21-GTPase-dependent dynamic activation complex within lipid rafts.

Pulkkinen K, Renkema GH, Kirchhoff F, Saksela K.

J Virol. 2004 Dec;78(23):12773-80.

2004

Cytomegalovirus retinitis in a patient treated with anti-tumor necrosis factor alpha antibody therapy for rheumatoid arthritis.

Haerter G, Manfras BJ, de Jong-Hesse Y, Wilts H, Mertens T, Kern P, Schmitt M.

Clin Infect Dis. 2004 Nov 1;39(9):e88-94. Epub 2004 Oct 11.

2004

Vpr and Vpu are important for efficient human immunodeficiency virus type 1 replication and CD4+ T-cell depletion in human lymphoid tissue ex vivo.

Rucker E, Grivel JC, Munch J, Kirchhoff F, Margolis L.

J Virol. 2004 Nov;78(22):12689-93.

2004

Comprehensive analysis of nef functions selected in simian immunodeficiency virus-infected macaques.

Schindler M, Munch J, Brenner M, Stahl-Hennig C, Skowronski J, Kirchhoff F.

J Virol. 2004 Oct;78(19):10588-97.

2004

Human cytomegalovirus tegument proteins ppUL82 (pp71) and ppUL35 interact and cooperatively activate the major immediate-early enhancer.

Schierling K, Stamminger T, Mertens T, Winkler M.

J Virol. 2004 Sep;78(17):9512-23.

2004

A naturally occurring variation in the proline-rich region does not attenuate human immunodeficiency virus type 1 nef function.

Rucker E, Munch J, Wildum S, Brenner M, Eisemann J, Margolis L, Kirchhoff F.

J Virol. 2004 Sep;78(18):10197-201.

2004

CD34 cell selection of peripheral blood progenitor cells using the CliniMACS device for allogeneic transplantation: clinical results in 102 patients.

Ringhoffer M, Wiesneth M, Harsdorf S, Schlenk RF, Schmitt A, Reinhardt PP, Moessner M, Grimminger W, Mertens T, Reske SN, Dohner H, Bunjes D.

Br J Haematol. 2004 Aug;126(4):527-35.

2004

Thrombocytopenia and acute renal failure in Puumala hantavirus infections.

Rasche FM, Uhel B, Kruger DH, Karges W, Czock D, Hampl W, Keller F, Meisel H, von Muller L.

Emerg Infect Dis. 2004 Aug;10(8):1420-5.

2004

Bacteremia in an immunocompromised patient caused by a commensal Neisseria meningitidis strain harboring the capsule null locus (cnl).

Vogel U, Claus H, von Muller L, Bunjes D, Elias J, Frosch M.

J Clin Microbiol. 2004 Jul;42(7):2898-901.

2004

Differential regulation of human immunodeficiency virus type 2 and simian immunodeficiency virus promoter activity.

Hiebenthal-Millow K, Pohlmann S, Munch J, Kirchhoff F.

Virology. 2004 Jul 1;324(2):501-9.

2004

Nef proteins from simian immunodeficiency virus-infected chimpanzees interact with p21-activated kinase 2 and modulate cell surface expression of various human receptors.

Kirchhoff F, Schindler M, Bailer N, Renkema GH, Saksela K, Knoop V, Muller-Trutwin MC, Santiago ML, Bibollet-Ruche F, Dittmar MT, Heeney JL, Hahn BH, Munch J.

J Virol. 2004 Jul;78(13):6864-74.

2004

Oligoclonal CD8+ T-cell expansion in patients with chronic hepatitis C is associated with liver pathology and poor response to interferon-alpha therapy.

Manfras BJ, Weidenbach H, Beckh KH, Kern P, Moller P, Adler G, Mertens T, Boehm BO.

J Clin Immunol. 2004 May;24(3):258-71.

2004

Amino acid 324 in the simian immunodeficiency virus SIVmac V3 loop can confer CD4 independence and modulate the interaction with CCR5 and
alternative coreceptors.

Pohlmann S, Davis C, Meister S, Leslie GJ, Otto C, Reeves JD, Puffer BA, Papkalla A, Krumbiegel M, Marzi A, Lorenz S, Munch J, Doms RW, Kirchhoff F.

J Virol. 2004 Apr;78(7):3223-32.

2004

The UL97 protein kinase of human cytomegalovirus and homologues in other herpesviruses: impact on virus and host.

Michel D, Mertens T.

Biochim Biophys Acta. 2004 Mar 11;1697(1-2):169-80.

2004

Hydroxymethyl-glutaryl coenzyme a reductase inhibition limits cytomegalovirus infection in human endothelial cells.

Potena L, Frascaroli G, Grigioni F, Lazzarotto T, Magnani G, Tomasi L, Coccolo F, Gabrielli L, Magelli C, Landini MP, Branzi A.

Circulation. 2004 Feb 3;109(4):532-6. Epub 2004 Jan 26.

2003

Constitutive signaling of the human cytomegalovirus-encoded receptor UL33 differs from that of its rat cytomegalovirus homolog R33 by promiscuous activation of G proteins of the Gq, Gi, and Gs classes.

Casarosa P, Gruijthuijsen YK, Michel D, Beisser PS, Holl J, Fitzsimons CP, Verzijl D, Bruggeman CA, Mertens T, Leurs R, Vink C, Smit MJ.

J Biol Chem. 2003 Dec 12;278(50):50010-23. Epub 2003 Sep 30.

2003

Alterations in HIV-1 LTR promoter activity during AIDS progression.

Hiebenthal-Millow K, Greenough TC, Bretttler DB, Schindler M, Wildum S, Sullivan JL, Kirchhoff F.

Virology. 2003 Dec 5;317(1):109-18.

2003

Polyoma virus-associated interstitial nephritis in a patient with acute myeloic leukaemia and peripheral blood stem cell transplantation.

Stracke S, Helmchen U, von Muller L, Bunjes D, Keller F.

Nephrol Dial Transplant. 2003 Nov;18(11):2431-3.

2003

Treatment of chronic hepatitis B with high-dose intravenous N-acetyl-L-cysteine.

Weidenbach H, Orth M, Adler G, Mertens T, Schmid RM.

Hepatogastroenterology. 2003 Nov-Dec;50(54):2105-8.

2003

Mutations in the C3 region of human and simian immunodeficiency virus envelope have differential effects on viral infectivity, replication, and CD4-dependency.

Otto C, Puffer BA, Pohlmann S, Doms RW, Kirchhoff F.

Virology. 2003 Oct 25;315(2):292-302.

2003

Down-modulation of mature major histocompatibility complex class II and up-regulation of invariant chain cell surface expression are well-conserved functions of human and simian immunodeficiency virus nef alleles.

Schindler M, Wurfl S, Benaroch P, Greenough TC, Daniels R, Easterbrook P, Brenner M, Munch J, Kirchhoff F.

J Virol. 2003 Oct;77(19):10548-56.

2003

Relationship between infectious burden, systemic inflammatory response, and risk of stable coronary artery disease: role of confounding and reference group.

Rothenbacher D, Brenner H, Hoffmeister A, Mertens T, Persson K, Koenig W.

Atherosclerosis. 2003 Oct;170(2):339-45.

2003

Enhanced CD4 down-modulation by late stage HIV-1 nef alleles is associated with increased Env incorporation and viral replication.

Arganaraz ER, Schindler M, Kirchhoff F, Cortes MJ, Lama J.

J Biol Chem. 2003 Sep 5;278(36):33912-9. Epub 2003 Jun 19.

2003

Constitutive inositol phosphate formation in cytomegalovirus-infected human fibroblasts is due to expression of the chemokine receptor homologue pUS28.

Minisini R, Tulone C, Luske A, Michel D, Mertens T, Gierschik P, Moepps B.

J Virol. 2003 Apr;77(8):4489-501.

2003

Human cytomegalovirus infection in human renal arteries in vitro.

Reinhardt B, Vaida B, Voisard R, Keller L, Breul J, Metzger H, Herter T, Baur R, Luske A, Mertens T.

J Virol Methods. 2003 Apr;109(1):1-9.

2003

Gene silencing by adenovirus-delivered siRNA.

Shen C, Buck AK, Liu X, Winkler M, Reske SN.

FEBS Lett. 2003 Mar 27;539(1-3):111-4.

2003

A nonconventional nuclear localization signal within the UL84 protein of human cytomegalovirus mediates nuclear import via the importin alpha/beta pathway.

Lischka P, Sorg G, Kann M, Winkler M, Stamminger T.

J Virol. 2003 Mar;77(6):3734-48.

2003

Viral escape and T-cell immunity during ganciclovir treatment of cytomegalovirus infection: case report of a pancreatico-renal transplant recipient.

Benz C, Holz G, Michel D, Awerkiew S, Dries V, Stippel D, Goeser T, Busch DH.

Transplantation. 2003 Mar 15;75(5):724-7.

2003

Identification of the first nonpeptidergic inverse agonist for a constitutively active viral-encoded G protein-coupled receptor.

Casarosa P, Menge WM, Minisini R, Otto C, van Heteren J, Jongejan A, Timmerman H, Moepps B, Kirchhoff F, Mertens T, Smit MJ, Leurs R.

J Biol Chem. 2003 Feb 14;278(7):5172-8. Epub 2002 Nov 26.

2002

Identification of the First Nonpeptidergic Inverse Agonist for a Constitutively Active Viral-encoded G Protein-coupled Receptor*

Paola Casarosa, Wiro M. Menge, Rosalba Minisini, Claas Otto, Jane van Heteren,Aldo Jongejan, Henk Timmerman, Barbara Moepps, Frank Kirchhoff, Thomas Mertens, Martine J. Smit, and Rob Leurs

J Biol Chem. 2003 Feb 14;278(7):5172-8. Epub 2002 Nov 26.

2002

Role of the Human Herpesvirus 6 U69-Encoded Kinase in the Phosphorylation of Ganciclovir

Leen de Bolle, Detlef Michel, Thomas Mertens, Chaysavanh Manichanh, Henri Agut, Erik de Clercq, and Lieve Naesens

Mol Pharmacol. 2002 Sep;62(3):714-21.

2002

High Variability between Results of Different In-House Tests for Cytomegalovirus (CMV) Monitoring and a Standardized Quantitative Plasma CMV PCR Assay

Lutz von Müller, Walter Hampl, Joachim Hinz, Helga Meisel, Angela Reip, Elisabeth Engelmann, Regine Heilbronn, Barbara Gärtner, Oliver Krämer, Hermann Einsele, Holger Hebart, Tatjana Ljubicic, Jürgen Löffler and Thomas Mertens

J Clin Microbiol. 2002 Jun;40(6):2285-7.

2001

Opinion Article: Cytomegalovirus is a Risk Factor in Atherogenesis

Barbara Reinhardt, Rosalba Minisini and Thomas Mertens

Herpes. 2002 Apr;9(1):21-3.

2001

Case report:Fatal outcome in a patient developing Epstein–Barr virus-associated lymphoproliferative disorder (EBV-LPD) without measurable disease

M Ringhoffer, K Döhner, S Schei, H Wilts, T Mertens, W Grimminger, H Döhner and D Bunjes

Bone Marrow Transplant. 2001 Sep;28(6):615-8.

2000

Aciclovir Selects for Ganciclovir-Cross- Resistance of Human Cytomegalovirus In Vitro That is Only in Part Explained by Known Mutations in the UL97 Protein

Detlef Michel, Simone Höhn, Tobias Haller, Dou Jun and Thomas Mertens

J Med Virol. 2001 Sep;65(1):70-6.

2000

Comparison between Human Cytomegalovirus pUL97 and Murine Cytomegalovirus (MCMV) pM97 Expressed by MCMV and Vaccinia Virus: pM97 Does Not Confer Ganciclovir Sensitivity

Markus Wagner, Detlef Michel, Peter Schaarschmidt, Bianca Vaida, Stipan Jonjic, Martin Messerle, Thomas Mertens and Ulrich Koszinowski

J Virol. 2000 Nov;74(22):10729-36.

gepunktete blaue Linie 1000px breit